Volume 15, Issue 3 p. 329-339

The effect of a synergistic concentration of a Piper nigrum extract used in conjunction with pyrethrum upon gene expression in Drosophila melanogaster

H. R. Jensen

H. R. Jensen

Department of Biology, University of Ottawa, Center for Advanced Research in Environmental Genomics, Ottawa, Ontario, Canada

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I. M. Scott

I. M. Scott

Department of Biology, University of Ottawa, Center for Advanced Research in Environmental Genomics, Ottawa, Ontario, Canada

Present address: Natural Health Products Directorate (NHPD), Health Canada, Ottawa, Canada.

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S. R. Sims

S. R. Sims

Whitmire Micro-General, 3568 Tree Court Industrial Blvd., St Louis, MO, USA

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V. L. Trudeau

V. L. Trudeau

Department of Biology, University of Ottawa, Center for Advanced Research in Environmental Genomics, Ottawa, Ontario, Canada

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J. T. Arnason

J. T. Arnason

Department of Biology, University of Ottawa, Center for Advanced Research in Environmental Genomics, Ottawa, Ontario, Canada

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First published: 31 May 2006
Citations: 30
John Thor Arnason, University of Ottawa, Department of Biology, 30 Marie-Curie Street, Ottawa, Ontario K1N 6N5, Canada. Tel.: (613)562 5262; fax: (613)562 5765; e-mail: [email protected]

Abstract

An ethyl acetate extract of Piper nigrum L. (Piperaceae) peppercorns was tested as a synergist for the botanical insecticide pyrethrum. A high synergist ratio of 11.6 against Drosophila melanogaster was obtained for the combination of pyrethrum supplemented with P. nigrum. The effect of this combination was investigated using cDNA microarray analysis of gene expression profiles in D. melanogaster. Treatment of D. melanogaster with pyrethrum alone resulted in a large number of differentially expressed genes, principally associated with stress responses. Seven genes were identified as being commonly expressed in D. melanogaster treated with at least two of the following treatments: P. nigrum, pyrethrum or P. nigrum plus pyrethrum. These are likely implicated in Drosophila defence responses to toxins.